为开发制作简便、花色丰富、致畸率低的试管花卉新产品，首先以单瓣矮牵牛品种梦幻(粉红脉纹)为模型，探究光周期、光照强度、温度、培养基蔗糖浓度及瓶内湿度等因素对花芽诱导与成花的影响，将优化后的条件应用于梦幻(粉红脉纹)、美声(紫红)、超级瀑布(红色)、泛美海市蜃楼(蓝色)、百万小铃(舞春花，橙色)和百万小铃(舞春花，黄色)等 6个不同花色品种，验证其普适性。结果表明，在含 40 g/L蔗糖的 MS培养基( pH 6.0)的三角瓶中，用振泰透气膜封口，在光周期 16 L∶8D、光照强度 2 000 lx、温度 25 ℃条件下培养，单瓣矮牵牛可在三角瓶中成功诱导花芽并正常开花，在瓶中连续开花，花期 30 d，6种不同花色品种均能完成成花过程，形成兼具多花色的微型景观。通过研究建立了一套稳定、普适的试管花卉标准化制作工艺，该工艺不依赖外源激素，操作可靠，同时为植物生殖发育与花色调控研究提供了理想模型。

To develop new in vitro flowering products that are easy to prepare, rich in flower color, and low in malformationrate, the single-petaled petunia variety Dream (pink-veined), was first used as a model to investigate the effects of photoperiod, lightintensity, temperature, sucrose concentration in the medium, and in-bottle humidity on flower bud induction and flowering. Subse.quently, the optimized and integrated protocol were applied to 6 differently colored varieties, including Dream (pink-veined), Melody(purple-red), Super Cascade (red), Pan American Mirage (blue), Million Bells (also known as Calibrachoa, orange), and Million Bells(also known as Calibrachoa, yellow) to validate its universality. Results showed that single-petaled petunia plants could successfullyinduced flower buds and bloomed normally in Erlenmeyer flasks when cultured in MS medium containing 40 g/L sucrose at pH 6.0,sealed with Zhentai gas-permeable membranes, under a photoperiod of 16 h light and 8 h dark, a light intensity of 2 000 lx, and a tem.perature of 25 ℃. The plants exhibited continuous flowering within the flask for up to 30 d. All 6 differently colored varieties success.fully completed the flowering process, forming miniature landscapes with multiple flower colors. This study established a stable anduniversal standardized protocol for producing in vitro flowering plants. The protocol is hormone-free, operationally reliable, and alsoprovides an ideal model for studying plant reproductive development and flower color regulation.

S681.9

国家现代农业产业技术体系( CARS-21)；甘肃省农业科学院科技成果转化项目( 2025GAAS-CGZH06、 2026GAAS-CGZH07)。