为研发低毒植物源绿色杀菌剂提供候选化合物，以制备的 15%厚朴酚乳油为试材，以土传病害致病菌立枯丝核菌( Rhizoctonia solani)为防治对象，系统测定厚朴酚不同有效成分含量对病菌细胞膜通透性、超氧化物歧化酶( SOD)活性及可溶性蛋白含量的影响。结果表明，厚朴酚乳油可显著破坏菌丝细胞膜完整性，厚朴酚乳油有效成分含量为 25.0 μg/mL处理 80 min时菌丝相对电导率达 26.66%，较空白对照提升 9.98个百分点，膜损伤程度随药剂浓度、处理时间升高而加剧；药剂胁迫诱导菌体氧化应激时，厚朴酚乳油有效成分含量为 10.0 μg/mL处理下 SOD活性高达 298.7 U/g，较空白对照提升 126.98%；胞内可溶性蛋白大量降解渗漏，同等有效成分含量可溶性蛋白仅 0.19 mg/g，较空白对照降低 97.44%。综上认为，厚朴酚乳油通过破坏细胞膜屏障、诱发持续氧化损伤、抑制胞内蛋白稳态多重途径抑制立枯丝核菌正常生理代谢，具备优良抑菌活性，在作物土传病害绿色防控领域开发前景广阔。

To provide candidate compounds for the development of low-toxicity botanical green fungicides, the prepared 15%magnolol emulsifiable concentrate (EC) was used as the experimental material, and the soil-borne pathogen Rhizoctonia solani was se. lected as the target organism. Effects of different magnolol concentrations on cell membrane permeability, superoxide dismutase (SOD)activity and soluble protein content of the pathogen were systematically determined. Results showed that magnolol EC significantly dis.rupted the integrity of the fungal cell membrane. When treated with magnolol EC at an active ingredient concentration of 25.0 μg/mLfor 80 min, the relative electrical conductivity of mycelia reached 26.66%, which was 9.98 percentage points higher than that of theblank control. The degree of membrane damage increased with the rise of medicament concentration and treatment time. Under medi.cament stress-induced oxidative stress, SOD activity reached 298.7 U/g under treatment with magnolol EC at an active ingredient con.centration of 10.0 μg/mL, representing a 126.98% increase compared with the blank control. Intracellular soluble protein was largelydegraded and leaked, with only 0.19 mg/g soluble protein detected at the same concentration, a 97.44% reduction compared with theblank control. In conclusion, magnolol EC inhibits the normal physiological metabolism of R. solani by multiple pathways includingdamaging cell membrane barrier, inducing persistent oxidative damage and disrupting intracellular protein homeostasis. It exhibits ex.cellent antifungal activity and has broad development prospects in green prevention and control of crop soil-borne diseases.protein

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甘肃省农业科学院科技成果转化项目( 2025GAAS-CGZH07-7)。