为了筛选适合于作物土传病害绿色防控中土壤消毒所用的生物熏蒸材料，选择 7个油菜品种 (系)陇 12PZ16-65、16QP-65、陇油 7号、陇油 8号、 R0162、R0153、R0157的种子作为指示材料，先进行种子硫代葡萄糖苷含量测定，然后选用硫代葡萄糖苷含量高于 100 μmol/g的油菜种子在室内采用熏蒸法测定其对立枯丝核菌( Rhizoctonia solani)、茄病镰孢菌( Fusarium solani)、辣椒疫霉菌( Phytophthora capsici)、大丽轮枝菌( Verti. cillium dahliae Kleb)及核盘菌[Sclerotinia sclerotiorum(Lib) de Bary] 5种病原真菌的菌丝抑制效果。测定结果表明， 7个油菜品种(系)的种子中，陇 12PZ16-65、16QP-65、陇油 7号、 R0162硫苷含量均高于 100 μmol/g，分别为 142.68、132.64、125.17、162.64 μmol/g；陇油 8号、 R0153、R0157分别为 68.87、40.79、42.08 μmol/g。室内抑菌效果测定结果表明， R0162对 5种病原菌均表现出较高的杀灭活性，抑制效果均最好，菌丝抑制率达 100%；陇 12PZ16-65、16QP-65、陇油 7号对立枯丝核菌的菌丝抑制率高峰值为 84.8%~94.8%；对核盘菌的菌丝抑制率高峰值为 82.1%～90.1%；对大丽轮枝菌的菌丝抑制率高峰值为 68.7%～77.3%；对茄病镰孢菌抑制效果差异较大，菌丝抑制率高峰值为 59.3%～77.7%；对辣椒疫霉菌的菌丝抑制率最高值仅 50.0%左右。因此，硫代葡萄糖苷含量不同的油菜种子对不同病原菌抑制活性不同，而 R0162则表现出广谱抑菌活性，可作为蔬菜土传病害防控中兼具生物熏蒸及绿肥作用的植物熏蒸材料，值得进一步研究利用。

To screen biofumigation materials suitable for soil disinfection in the green control of crop soil-borne diseases,seeds of 7 rape varieties or lines, namely Long12PZ16-65, 16QP-65, Longyou 7, Longyou 8, R0162, R0153, and R0157, were select.ed as experimental materials. The glucosinolate content of the seeds was first determined, and rape seeds with glucosinolate contentshigher than 100 μmol/g were then selected for indoor fumigation assays to evaluate their inhibitory effects on the mycelial growth of 5pathogenic fungi: Rhizoctonia solani, Fusarium solani, Phytophthora capsici, Verticillium dahliae Kleb, and Sclerotinia sclerotiorum (Lib) de Bary. Results showed that among the seeds of the 7 rape varieties or lines, Long12PZ16-65, 16QP-65, Longyou 7, and R0162had glucosinolate contents higher than 100 μmol/g, reaching 142.68, 132.64, 125.17, and 162.64 μmol/g, respectively, whereas Longy.ou 8, R0153, and R0157 had glucosinolate contents of 68.87, 40.79, and 42.08 μmol/g, respectively. The indoor antifungal assayshowed that R0162 exhibited high fungicidal activity against all 5 pathogens and had the strongest inhibitory effect, with a mycelial in.hibition rate of 100%. Long12PZ16-65, 16QP-65, and Longyou 7 showed peak mycelial inhibition rates of 84.8% to 94.8% against R. solani, 82.1% to 90.1% against S. sclerotiorum, and 68.7% to 77.3% against V. dahliae. Their inhibitory effects against F. solani varied considerably, with peak mycelial inhibition rates of 59.3% to 77.7%, while the highest mycelial inhibition rate against P. capsici was only approximately 50.0%. Therefore, rape seeds with different glucosinolate content showed different inhibitory activities against dif.ferent pathogens, while R0162 showed broad-spectrum inhibition fungal activity. R0162 could be used as a material with biological fu.migation and green manure in the prevention and control of vegetable soil-borne diseases.

S565.4；S435.65

甘肃省科技计划( 24CXNA025)；国家重点研发计划( 2022YFD1401200)。