建立环保高效的楮实子多酚提取工艺并探究其抗氧化活性，为楮实子的开发应用提供实验支持。以楮实子为原料，采用超声波辅助溶剂法提取多酚，以多酚得率为核心指标，在单因素试验基础上，通过响应面法对提取工艺进行优化，并借助 DPPH自由基清除法与铁氰化钾还原法测定提取物的抗氧化活性。结果表明，楮实子多酚的最佳提取工艺参数为液料比 53∶1 mL/g、乙醇体积分数 43%、超声功率 180 W、浸提时间 125 min、超声温度 45 ℃，浸提次数 2次。在此条件下，多酚得率可达(6.26±0.32) mg/g。抗氧化活性验证显示，楮实子多酚具有较强的自由基清除能力与还原活性。超声波辅助溶剂法兼具高效提取多酚与保留其优良抗氧化活性的优势，具备良好的应用潜力。

This study aimed to establish an environmentally friendly and efficient extraction process for polyphenols fromBroussonetia papyrifera fruits and to investigate their antioxidant activity, thereby providing experimental support for the development and utilization of Broussonetia papyrifera fruits. This study employed the ultrasonic-assisted solvent extraction method using Brousson. etia papyrifera fruits as raw materials. With polyphenol yield as the core index, the extraction process was optimized via response sur.face methodology based on single-factor experiments. Meanwhile, the antioxidant activity of the extracts was determined by means ofDPPH free radical scavenging assay and potassium ferricyanide reduction method. Results showed that the optimal extraction parame.ters for polyphenols from Broussonetia papyrifera fruits were as followed: liquid-to-solid ratio of 53:1 (mL/g), ethanol volume fractionof 43%, ultrasonic power of 180 W, extraction time of 125 min, ultrasonic temperature of 45°C, and two extraction cycles. Under theseconditions, the polyphenol yield could reach 6.26±0.32 mg/g. Antioxidant activity tests confirmed that the polyphenols from Broussone. tia papyrifera fruits exhibited strong free radical scavenging ability and reducing activity. In conclusion, the ultrasonic-assisted sol.vent extraction method has the advantages of efficiently extracting polyphenols while preserving their excellent antioxidant activity,thus demonstrating good application potential.pacity

TS201.4

甘肃省农业科学院重点研发计划项目(2023GAAS14)。