以全叶苦苣菜种子和植株为材料，进行外植体的灭菌、植株再生体系建立研究。结果表明，全叶苦苣菜的种子和茎段经过灭菌，接种到1/2MS培养基上可获得无菌苗。用于愈伤组织诱导最佳的材料为无菌苗下胚轴，最佳培养基为MS+0.5 mg/L BA +1.5 mg/L 2，4-D，愈伤组织分化最佳培养基为MS+ZT 2.0 mg/L +IAA 0.5 mg/L，分化出的不定芽在培养基1/2MS+IAA 0.2 mg/L上生根效果较好，可以获得健壮再生植株。试管苗移栽到温室，成活率可达到95%以上。移栽后植株的植物学性状和野生植株基本一致。

The seeds and plants the wild resources of Sonchus transcaspicus Nevski were used as materials to do the reseach on sterilization of explants， establishment of plant regeneration system. The results demonstrated that the seeds and stems of Sonchus transcaspicus Nevski are sterilized and inoculated on 1/2MS medium to obtain sterile seedlings. The most suitable material for callus induction is hypocotyl， and the ideal medium is MS+0.5 mg/L BA +1.5 mg/L 2，4-D. The ideal medium for callus differentiation is MS+ZT 2.0 mg/L + IAA 0.5 mg/L， The differentiated cluster buds can be proliferated and cultured by stem cutting. The differentiated adventitious buds have a better rooting effect on the medium 1/2MS+IAA 0.2 mg/L and healthy regenerated plants can be obtained. When the test-tube seedlings are transplanted into a nutrient bowl in greenhouse， the survival rate can reach more than 95%. The tube seedlings stable planted maintained all botany characteristics of the wild Sonchus transcaspicus Nevski.

S647

兰州市人才创新创业项目“苦苣菜引种驯化及新品种选育”(2017-RC-55)；中国农业科学院基本科研业务费院级统筹专项“寒生旱生灌草资源引种驯化及新品种示范”(Y2018PT77)。